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Description
Following these procedures, the cell pellets of each sample were collected by centrifugation at various durations (6, 12, 24, 48 h) and resuspended in binding buffer (20 mM NaH 2 PO 4 , 500 mM NaCl, 30 mM imidazole, pH 7.4)
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doi: 10.1007/978-3-319-15126-7_38
This disruption lowers melatonin and alters GnRH secretion, affecting the hypothalamic-pituitary-ovarian (HPO) axis
Greger survives deadly smoothie"
