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Materials and Methods Cloning, Site-Directed Mutagenesis, Protein Expression, and Purification Sequences coding for PtGSTU19 (Potri.008G174900) and PtGSTU20 (Potri.008G175000) were amplified by PCR from poplar cDNA using specific primers (Supplementary Table S1) and cloned into pET-12a plasmid (Novagen) between Nde I and Bam HI restriction sites

Research has identified two mechanisms by which lipid hydroperoxides are generated: one involves the peroxidation of PUFA-PLs [41], while the other is directly synthesized by lipoxygenases [42]

The surface becomes less reflective, and that plump, glassy finish we associate with youth starts to flatten

and Fryer, A.A

[PubMed: 11451741] 262
