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ReverTraR Ace qPCR RT Master Mix with gDNA Remover and Blend TaqR was utilized to produce cDNA and to amplify the synthesized cDNA (Toyobo Co., Ltd., Science Department, Osaka, Japan)

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When co-stained for P-Cadherin, the ProtK step was omitted, and after the last three washes in PBS, slides were blocked for 2 h in 10% HISS in PBS