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This triple-receptor approach positions Retatrutide as the most mechanistically complex metabolic peptide currently in active research , building on the dual-receptor framework of Tirzepatide by adding glucagon receptor co-activation as a third pathway

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Materials and methods Cloning, expression, and purification of Syn constructs Human Syn WT (#36046), Syn WT -141C (#108866), and Syn S129E (#36050) constructs were obtained from Addgene