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PMID 15483636
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Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas
Standard reconstitution is 5mg powder + 1mL bacteriostatic water, yielding 5mg/mL concentration
