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The hypoxia alleviation via O2 production, the redox balance disruption via glutathione consumption and the reactive oxygen species generation, together would down-regulate the expression of O6-methylguanine-DNA methyltransferase under TMZ medication, which is considered as the key to drug resistance

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Key Vocabulary Terms Term Definition Affinity tag Peptide or protein tag used to purify other proteins Ligand Ion, small molecule, peptide, or protein that binds to an affinity tag Reactive Group Chemical moiety used to bind ligands to agarose Spacer Linker between the agarose bead and reactive group / ligand Activated Agarose is activated when a reactive group and spacer are chemically added to it Affinity Purification Affinity purification is a common technique that leverages naturally occurring interactions to enable protein purification

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