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For example, vitamin D deficiencies are often treated with high-dose vitamin D injections or supplements that deliver over 50,000 IU of vitamin D, which is much more than the UL ( Though most supplement bottles provide recommendations regarding how much of a vitamin to take per day, needs can vary from person to person

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After 24 and 48 h, cells were fixed using 4% paraformaldehyde (PFA), permeabilized with 0.2% Triton X-100 in PBS, and blocked with 2% BSA