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Mushrooms are considered spoiled when water loss reaches 5% of their original fresh mass (44)
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Ultimately, Health Canada (HC 2018) selected the BMDL associated with an extra risk of 5% for hepatocellular necrosis in the combined male and female rat data set as the basis for deriving the TDI (BMDL 5 = 5.4 mg/kg/d for males and females combined)
When OD600 was increased to 0.6~0.8, isopropyl D-1-thiogalactopyranoside (IPTG) (Sigma, St
