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Folate insufficiency was one of these three variables that had the most impact on the serum tHcy levels, followed by MTHFR C677T and vitamin B12 deficiency (in decreasing order of impact) [48]

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Limit of quantification (LOQ) was expressed as: LOQ = 10 SD S 1 , where S is the slope of the calibration curve and SD is the standard deviation of the response