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doi: 10.1111/j.1600-0781.2006.00220.x

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Validation of RNA-Seq by Quantitative Real-Time PCR(qRT-PCR) Total RNA (2 g) from three biological replicates was purified and reverse-transcribed into cDNA using TranScript One-step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China) with oligo(dT)

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To visualize the proteomic bands, an enhanced western luminescent detection kit (Vigorous Biotechnology, Beijing, China) was employed