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Control culture variables that affect signaling: Passage number, confluence, serum concentration, extracellular matrix coating, oxygen condition, mycoplasma status, baseline cell health, and image-analysis thresholds can all influence FAK phosphorylation, VEGFR2 readouts, and tube-formation results
The average American diet contains 5-15 mcg per day according to NHANES studies
doi: 10.1096/fj.03-0782fje
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