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Biohacker Protocol (experimental) Dose: 0.25 mg (= 10 clicks) Duration: Continuous Frequency: Twice daily Cycle Interval: No fixed interval / take 1 week off every 8 weeks Goal / Description: Micro-dosing approach focusing on steady fat metabolism

Tracking redox shifts helps optimize culture conditions, maximize yield, and assess product stability

For preparation of the hot water extracts (WE), 5 g of spinach powder was put in a glass bottle with 100 mL of deionized water, extracted with an autoclave, and freeze dried (Ilshin, Yangju, Korea)

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