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Cells were then resuspended in 10 ml of cold PBS and incubated with cOmplete mini EDTA-free protease inhibitor (Roche, 11836170001) for 30 min at 4C

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monocytogenes gshF mutants are more sensitive to redox stressors such as hydrogen peroxide and diamide and are 200-fold less virulent in mice, indicating that bacterially-derived glutathione is essential for pathogenesis [17]