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CaCl 2 was added to achieve a final concentration of 1 mM, followed by treatment with PBS or OP (0.5 and 1 mg/mL) for an additional 30 min at the same temperature

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Commonly referenced reconstitution solutions include bacteriostatic water, sterile water, acetic acid and DMSO

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Wang Y., Xu M., Transl Neurosci 2021, 12, 210

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