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Description
The supernatants were transferred to new microtubes and analyzed by western blot
ECG: Electrocardiogram
Conditioned medium was harvested, filtered through 0.45-m filters, concentrated by ultracentrifugation (81550 g, 2 h at 4 C) and stored at 80 C
Acknowledgements The authors would like to thanks Prof
The autocrine role of placental extracellular vesicles from missed miscarriage in causing senescence: possible pathogenesis of missed miscarriage
