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Respiration and mitochondrial function analysis Mitochondrial function in cells treated with vehicle (BSA) or palmitate was assessed as previously described [15] and respiratory analysis of skeletal muscle biopsies was also performed as previously described [11]

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In reference to calpain during disuse muscle atrophy, it is clear that skeletal muscle inactivity promotes an increase in both cytosolic calcium levels and calpain activation (214, 241, 357, 379, 387)

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