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PCR was performed in an ABI StepOnePlus Real-Time PCR System (Applied Biosystems) under the following conditions: denaturation at 95 C for 15 s, followed by annealing and extension at 60 C for 30 s (40 cycles)

doi: 10.5811/westjem.2020.1.45944

Skin lightening treatment aims to reduce dark spots, pigmentation, and uneven skin tone to achieve a brighter complexion

Some of the natural non-enzymatic antioxidants found in skin to help neutralize the free radicals are L-ascorbic (vitamin C), alpha-tocopherol (vitamin E), beta-carotene, uric acid, Co-Q-ten, and glutathione

[DOI] [PMC free article] [PubMed] [Google Scholar] 91.Huang X., He Y., Dubuc A.M., Hashizume R., Zhang W., Reimand J., Yang H., Wang T.A., Stehbens S.J., Younger S., et al