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Comparison between groups was made using one-way analysis of variance (ANOVA) followed by post hoc Tukey HSD test or non-parametric Kruskal-Wallis test for multiple independent samples with post hoc test by the methods of Conover

The cells were incubated overnight at 4 C with one of the following primary antibodies: rabbit anti-SMAD2 (Cell Signaling Technology, Danvers, MA, RRID: AB_823638, 1:200 dilution), rabbit anti-SMAD3 (Cell Signaling Technology, RRID: AB_2193182, 1:200 dilution), rabbit anti-phospho-SMAD2 (Abcam, RRID: AB_2732791, 1:100 dilution), or rabbit anti-phospho-SMAD3 (Cell Signaling Technology, RRID: AB_2193207, 1:100 dilution)

Notably, microglia can induce a neurotoxic astrocytic phenotype by releasing IL-1, TNF-, and complement component C1q

TYPES OF PEPTIDES Common Uses: Injury recovery, gut health, reducing inflammation

Treatment Plan We agree on the areas to treat and the dose required for natural movement