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Preparing PT-141 nasal spray Why nasal spray: Avoids injections (needle-phobic) More convenient Slightly faster onset (30-60 min quicker) Lower bioavailability (~30% vs ~100% injection) Need higher dose to compensate Nasal spray supplies needed: Empty nasal spray bottle (10-15ml) Reconstituted PT-141 (higher concentration recommended) Syringe for transferring Small funnel (optional) Concentration for nasal spray: Higher concentration better (less volume per dose) Reconstitute 10mg PT-141 in 1ml water = 10mg/ml Or even 10mg in 0.5ml = 20mg/ml (very concentrated) Allows smaller spray volume Fewer sprays needed per dose Nasal spray dosing: Inject: 1.5mg dose Nasal equivalent: 3-5mg (lower absorption) 0.3ml of 10mg/ml concentration = 3mg nasal 0.5ml of 10mg/ml = 5mg nasal Typical: 2-3 sprays per nostril (0.1ml per spray) See complete PT-141 nasal spray guide for men for detailed protocols

32, 33 This hypothesis is supported by observations that the first-degree relatives of Crohns disease (CD) patients are at increased risk of developing IBD and some of these relatives also demonstrate mucosal leakiness

If reactions persist or worsen, it could indicate an issue with the reconstituted solution, the injection technique, or an allergy to a component like benzyl alcohol