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Secondly, the refolding process necessitates high concentrations of denaturants (e.g., urea, guanidine hydrochloride) and other reagents 22 , and its efficiency often decreases with increasing protein concentration, limiting scalability for large-scale production 23
Furthermore, improper activation of protein kinase C and Rho can modulate the actin cytoskeleton and influence tight junction regulation and function [158]

The American Journal of Tropical Medicine and Hygiene

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