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10.3945/ajcn.112.053132 14

Intramuscular injection Find the injection site

Moreover, a long-distance enhancer region 31 kb upstream of the NBEAL2 gene was discovered in data from ENCODE for K562 cells that express high levels of GATA1 and whole genome ChIP sequencing data for GATA1 and GATA2 binding sites using primary MKs (Figure 4).35 Typical WGATAR recognition sequences (GATA1/2 BS) are present in this region, and a physical interaction between this enhancer region and the NBEAL2 promoter was predicted based on ChIA-PET experiments using K562 cells.43 It is known that long-distance enhancers come into close proximity of target promoters through looping.44 Transcription factors and their complexes mediate such enhancer-promoter loop formation

The high lipophilicity of PTER allows it to easily cross the blood-brain barrier (Temsamani et al., 2015), resulting in greater neuroprotection than RES (Choo et al., 2014)