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To reduce nausea and exhaustion, corticosteroids such as dexamethasone may be prescribed starting 3 days prior to each treatment

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Heat-induced epitope retrieval (HIER) was performed in a pressure cooker for 20 min using either citrate-based buffer at pH 6.0 (VectorLab, H-3300-250) or tris-based buffer at pH 9.0 (VectorLab, H-3301-250), according to the antibody manufacturers instructions