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The intense research in this field seen over the last few decades, has resulted in the discovery of several proteins that can upregulate the bone healing response [14, 15]

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Protein concentration was determined using a DC Protein Assay Kit, (Bio-Rad, United States)

These markers are inexpensive to track through routine bloodwork and provide an objective measure of hepatic response to the protocol

Overall, the dual-crosslinked polysaccharide hydrogels offered a novel approach to creating wound dressings in the future of therapeutic wound treatment