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2019;18:e12876

For this controversial issue, organoids may represent a long-awaited alternative to animal experiments

[47] the human colorectal cancer HCT116 cell line is used in its wild type and p53 -/ p53 - versions

For determination of the COX product 12( S )-hydroxy-5- cis -8,10- trans -heptadecatrienoic acid (12-HHT), freshly drawn human blood (2 ml) was pre-incubated with the indicated compounds at 37C for 10 min, and formation of 12-HHT was initiated by addition of 30 M Ca 2+ -ionophore A23187 plus 100 M AA

[,] Twelve years later, irisin, the cleaved form of fibronectin type III domain-containing 5 (Fndc5), had been discovered as a PGC1--dependent myokine that drives brown-fat-like development of the white fat and thermogenesis