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Membranes were washed again 3x for 10 min, treated with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher, 34095) and chemiluminescence acquired with a ChemiDoc MP imaging system (Bio-Rad)

To counteract these oxidants, cells have developed antioxidant systems, including antioxidant enzymes, for example, superoxide dismutases (SOD13), catalase, glutathione peroxidases (GPx18), thioredoxins (Trx12), and peroxiredoxins (Prx16)

The endothelium undergoes a significant transformation in metabolic syndrome, shifting to a dysfunctional state

doi: 10.1016/j.tips.2011.05.002

It replenishes essential fluids and electrolytes for optimal hydration, with a boost of B-complex vitamins for energy