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Disagreements were solved by the consensus of a third author (F.F.) The studies had to include individuals with a clinical diagnosis of any type of dementia and severity according to the internationally accepted guidelines or diagnostic criteria

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As the enzyme was present in a storage solution composed of 50% glycerol solution containing ~9 mg/mL bovine serum albumin, 30% sucrose, 1.5 mM EDTA, 1.5 mM EGTA, 1.5 mM 2-mercaptoethanol, 0.3 TRITON X-100, 0.003% sodium azide, and 15 mM potassium phosphate, pH 6.8, the storage solution was replaced with 50 mM MOPS/HCl pH 7.4 prior use