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The monocytes (2x10 6 )/well were seeded in wells of six-well plates and cultured in a nutrient-rich culture medium (RPMI1640 medium supplemented with 10% calf serum, 2 mM mercaptoethanol, 1 mM glutamine, 100 unit/ml penicillin G, and 100 g/ml streptomycin sulphate) and a DC growth stimulant mix of GM-CSF and IL-4

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(2015) suggested that where neurons are too damaged to use the lactate produced from glucose by astrocytes (i.e., by uncoupling of neuronal and glial metabolism), high extracellular levels of lactate would accumulateexplaining the association between high lactate and poor outcome