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Journal of Molecular Medicine

Furthermore, the gliotoxin treatment also reduced the DNA-binding activity of NF-B, thus impeding the inflammation mediated by NF-B activation in the colon [30]

After standing at room temperature for 30 min, the mixture was heated (80 C) until a clear solution was obtained

Mutations were introduced by site-directed mutagenesis

It's a crucial distinction and a common source of frustration
