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We therefore cotransfected hESCs with the EGFP reporters and PUM2::FLAG (Figure 6A), and we found that PUM2::FLAG dramatically repressed Erk2 3UTR(wt) expression (Figure 6F, 6G, and 6J) but did not repress Erk2 3UTR(mut) expression (Figure 6H, 6I, and 6J)