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Antagonism of the ATP-gated P2X7 receptor: a potential therapeutic strategy for cancer

Glutathione also plays a vital role in the recycling of other important antioxidants such as vitamins C and E, so their net protective effects are increased

doi: 10.3390/PHARMACEUTICS14081601 [DOI] [PMC free article] [PubMed] [Google Scholar] 182.Romn-Aguirre M, Leyva-Porras C, Cruz-Alcantar P, Aguilar-Elguzabal A, Saavedra-Leos MZ

Protein concentration was determined using a BCA protein assay kit (Thermo Fisher Scientific), and 20 g of protein per sample (the default amount if not specified otherwise) were subjected to SDS-PAGE, transferred onto PVDF membranes, and incubated with primary antibodies summarized in the key resources table, including GLUT1 (1:1000), MCT1 (1:1000), LDHB (1:1000), SOX2 (1:1000), GPR81 (1:1000), DNMT3B (1:1000), H3 (1:1000), H3K27m3 (1:1000), COX IV (1:1000), TOMM20 (1:1000), Na + -K + ATPase (1:1000), LAMP1 (1:1000), GM130 (1:1000), GRP78 (1:1000), -Tubulin (1:5000), and anti-CD147 (HAb18, 1:3000) produced in our laboratory 54,66

& Philofsky, A