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To understand the underlying mechanisms, inhibitors targeting the Notch pathway (DAPT) and NF-B pathway (BMS) were also applied to HUVEC and murine CLI models

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Puzuka A, Alksere B, Gailite L, Erenpreiss J

The supernatant was injected into a C18 LiChroCart column (5 mm x 250 mm x 4 mm) and the mobile phase applied at a flow rate of 1 mL min 1 , consisting of 50 mM sodium phosphate (pH 2.8), 10 mM heptane sulfonate and 10% acetonitrile

FOXO4-DRI works by disrupting the interaction between the FOXO4 protein and p53, enabling p53 to activate apoptosis in senescent cells