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Loren Pickart, who noticed that when plasma from young individuals was added to the liver tissue of older individuals, the older tissue would produce proteins more characteristic of younger individuals

BV-2 cells were first seeded at a density of 1 10 5 cells/well in a 24-well plate for 24 h, pretreated with the test agents (equivalent to 18 M ASX) for 6 h, and incubated with medium containing 500 ng/mL LPS, except for the control group

Toxicol Sci 91 , 365371, doi: 10.1093/toxsci/kfj165 (2006)

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