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Zeng CW

Decades of clinical data confirm that

Ishikawa M, Takayanagi G, Sasaki K (1988) Effect of buthionine sulfoximine, an inhibitor of glutathione biosynthesis, on the selenium-induced lethality in mice

However, clinical trials have shown that xCT inhibitors are ineffective in patients with glioma and have numerous SASP-related side effects, which highlights the need for further clinical trials in patients with high tumor burdens (90)

Finally, after washing 3 times in PBT for 30 min each at room temperature and two times in alkaline-phosphatase (AP) buffer (100 mM Tris-HCl, pH 9.5, 100 mM NaCl, 0.1% Tween20, 50 mM MgCl 2 ) for 5 min each at room temperature, the slides were incubated with 1 L 75 mg/mL NBT and 3.5 L 50 mg/mL BCIP per mL AP buffer and developed in the dark for 2-20 h