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68 PadmanabhanN.UshijimaT.TanP

7.5 Challenges and controversies in researching the gut-brain-metabolic axis Despite the promising findings regarding the role of gut microbiota in regulating insulin resistance and cognitive function, several challenges and controversies remain in this field: 7.5.1 Variability in microbiota composition Individual variations in gut microbiota composition can lead to inconsistent results across studies

Methyl donor supplementation could modify DNA methylation and subsequent changes in genes responsible for reproducing enzymes involved in de novo hepatic lipogenesis, such as fatty acid synthase 28

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