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Glutathionylation of M pro at pH 7.5 and pH 6.8 To prepare glutathionylated M pro for use in analytical ultracentrifugation, SEC and activity assays, M pro was first exchanged into a buffer containing 40 mM tris-HCL, 2 mM EDTA and 300 mM NaCl at Ph 7.5 using Amicon 10 kDa cutoff filter units

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Furthermore, phenformin was not active in vitro against RSV and IAV

Wahane, Dept