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Neurological diseases had become the leading cause of DALYs, second only to cardiovascular diseases, and a major global determinant of mortality (15)

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Aliquoting Protocol Decide on a volume per aliquot based on your typical experiment size (e.g., 50100 L per aliquot) Use low-binding microcentrifuge tubes (protein low-bind or equivalent) Label each tube: compound name, concentration, date, lot number Flash freeze aliquots in liquid nitrogen or dry ice/ethanol bath before transferring to -80C storage Discard any aliquot that has been thawed and refrozen more than once See: Optimal Storage and Stability Guidelines for IGF-1 LR3 Lyophilized Research Peptide for detailed post-reconstitution storage guidance

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10.1371/journal.pone.0014564 [DOI] [PMC free article] [PubMed] [Google Scholar] Mancias JD, Wang X, Gygi SP, Harper JW, Kimmelman AC (2014) Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy