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Buffers with pH 5, 7, 9 were prepared by adjusting the pH of PBS buffer using 0.2 mol/L HCl or NaOH solution
The NAPstar3b variant was generated by site-directed mutagenesis on the pDONR207 NAPstar3 plasmid using the primer pair Pr1/Pr2 (Supplementary Table 6)
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2013;9 doi: 10.1371/journal.pgen.1003801
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