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Adherent cells were scrapped, then centrifuged at 4C and 13000 g for 15 min, and the resulting supernatants processed using established protocols.[19] Intracellular levels of 1-MNA and as well as the IS were determined from LC/MS/MS peak areas
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We recommend that you take both at least two hours apart

Thus, vitamin B12 deficiency results in the accumulation of methylmalonyl-CoA that is subsequently converted to methylmalonic acid (MMA), whose plasma levels are often elevated in patients with vitamin B12 deficiency
