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CRISPR-dCas9 epigenome editing platforms employ catalytically dead Cas9 fused to epigenetic effectors ( TET1 for active demethylation, DNMT3A for targeted methylation) without DNA breaks, enabling precise chromatin manipulation

In whole-cell current clamp experiments, hyperpolarizing or depolarizing current pulses (500 ms, from 10 to 30 pA in 5-pA steps) were injected into neurons to determine input resistances or trigger action potentials

Wilson, David A
